Possible targeted therapy approaches for CCA, like inhibiting the tyrosine kinase with Ceritinib (91), or the inhibition of the cyclin-dependent kinases 4 and 6 (92), of cell division cycle protein 20 (93), of long non-coding RNA LINC00665 (94), of mTORC1 (95), of CCR5 (96), and of protein SUMOylation (97), were studied using spheroids. This evidence concerns the gene CDK4 and cholangiocarcinoma.