Among the novel variants identified in this study, three of them haven’t been previously reported: so, we classified them using ACMG guidelines, assisted by VarSome (38), and uploaded their information into ClinVar (28) We also uploaded another variant in MLH1, not listed in ClinVar (p.Met621Ilefs*16,SCV005329258), identified in a patient who had colorectal cancer at the age of 36, whose father had three surgeries due to three independent colorectal tumors and died aged 54, with no other family members affected to date. This evidence concerns the gene MLH1 and colorectal neoplasm.