NFS1 was positively correlated with tumor purity (r = 0.124; P = 1.55 × 10−2) and negatively correlated with CD8+ T cells (r = −0.19; P = 2.06 × 10−4), CD4+ T cells (r = −0.133; P = 9.33 × 10−3), myeloid dendritic cells (r = −0.102; P = 4.63 × 10−2), and activated NK cells (r = −0.238; P = 2.79 × 10−6). Here, CD4 is linked to neoplasm.