We also found an increase in GFAP staining following CA/CPR (Sham: 3.227% ± 0.2149% vs. CA/CPR: 12.74% ± 2.303%, p = 0.0021; Figure 1C), suggesting astrocytes remain persistently activated at subacute timepoints after ischemia. This evidence concerns the gene GFAP and ischemia.