Immunoblotting and qRT-PCR assay found that CDCA3 mRNA and CDCA3 protein were overexpressed in PC cell lines compared with that in HPDE6-C7 cell line, and were further increased in chemoresistant cell lines compared with that in parental non-resistant cell lines (Figure 8C). Here, CDCA3 is linked to pachyonychia congenita.