In this study, it was found that PGK1 and SLC2A1 were highly expressed in TU212HPV cells and were enriched in the HIF-1 signaling pathway, indicating that PGK1 and SLC2A1 play important roles in maintaining the energy metabolism of laryngeal cancer cells, adapting to hypoxic conditions and promoting tumor development. This evidence concerns the gene PGK1 and laryngeal carcinoma.