To investigate the potential effects of CRELD2 on the malignant biological behavior of ESCC cells in vitro, the pcDNA3.1-CRELD2 plasmid and si-CRELD2 were respectively employed to upregulate or downregulate the expression of CRELD2 in KYSE150 and TE1 cells (Figure 3A). Here, CRELD2 is linked to esophageal squamous cell carcinoma.