To obtain a more comprehensive view regarding CARtein-mediated signaling activation in T-cells, we performed a two-way ANOVA analysis, comparing NFAT, NFκB and CD69 MFI values in CARtein-TPR cells when stimulated with MM.1S multiple myeloma cell line, which endogenously expresses BCMA and SLAMF7 proteins, or K562 cells, which have been engineered to express both simultaneously (Figures 10A, B). Here, CD69 is linked to AL amyloidosis.