In the present study, transcriptome analysis identified 28 DE-TrpGs in ESCC compared with normal samples, including eight upregulated (STAT1, IL4I1, TDO2, NMNAT3, KMO, IDO1, OGDHL, and ACAT2) and 20 downregulated DE-TrpGs (ALDH9A1, ALDH3A2, ADH1B, AOX1, MAOA, ECHS1, ALDH2, ACAT1, HAAO, ALDH7A1, HADHA, MAOB, AKR1A1, CYP4X1, INMT, CYP2U1, CAT, HADH, NMNAT1, and UGT2B11). Here, ALDH9A1 is linked to esophageal squamous cell carcinoma.