CERS1 and colorectal carcinoma: Among the analysed sphingolipids, weobserved a reduction in the expression of C18 CER.Considering that de novo ceramide synthesis has previouslybeen suggested as a key mechanism involvedin nCRT-induced apoptosis, our finding may resultfrom impaired activity of ceramide synthase 1, whichis responsible for C18 CER production [7], [22].Further research should investigate the levels andactivity of ceramide synthases and the activity ofASAH1, given that it has been shown to influencesensitivity to radiation therapy in CRC [23].