HMGB1 and alkaline phosphatase measurement: Using the joint detection Log(P)=-1.736+(0.645× HMGB-1)+(-0.454×AChE), it was found that HMGB-1+AChE had a sensitivity of 48.44% and a specificity of 88.33% for the diagnosis of AP (P<0.05, cut-off >0.639, Figure 1).