CDH17 and neoplasm: Spheroid dissociation mimics tissuedissociation, a model anticipating in vivo cell invasion. Metastatic cascade steps include angiogenesis,cadherin- and catenin-mediated tumor cell dissociation, and invasionthrough the tumor epithelium’s extracellular matrix. In this study, spheroid dissociation in thecontrol group may reflect natural processes in 3D cultures, such asnutrient and oxygen depletion, leading to reduced cohesion and structuralbreakdown over time.