The downregulation of MMP2 specifically in spheroids, but not in monolayer cultures, indicatesthat EGCG may inhibit metastatic potential in tumor-like environmentsby targeting extracellular matrix remodeling. This effect may be mediated through chromatin remodeling, as EGCGalso modulated the expression of genes such as HDAC1 in spheroids and EZH2, DNMT3A,and KDM1A in monolayers., These epigenetic regulators influence gene accessibility and mayindirectly regulate transcriptional programs involved in migrationand invasion. The gene discussed is DNMT3A; the disease is neoplasm.