NANOG and neoplasm: In PrEC and CWR cell lines, neither NANOG nor OCT4 expression was detected, and SOX2 expression was not associated with the up-regulation of stemness markers, enhancement of tumor sphere-forming ability, or induction of embryonic stem cell-related SOX2 target gene expression23 Further, chromatin immunoprecipitation-sequencing analysis for SOX2 in embryonic stem cells and CWR cells revealed that the primary gene targets regulated by SOX2 in PCa cells were almost entirely distinct from its targets in embryonic stem cells.