Although our results found that RAB26 could affect the stemness of PCSCs, activate the ERK signaling pathway, up-regulate the expression of TWIST1, and form a positive feedback regulation between the ERK signaling pathway and TWIST1 to enhance EMT and promote the malignant progression of PCa, we did not further explore the specific phosphorylation site of ERK. The gene discussed is TWIST1; the disease is posterior cortical atrophy.