NOX1 and neoplasm: Interestingly, green fluorescence from HSC-GFP decreased after treatment with setanaxib, which may be due to the inhibition of NOX1 previously described function on fibroblast proliferation.34 The inactivation of fibroblasts together with reduced proliferation may impact the crosstalk of tumour cells and fibroblast since both spheroid size and red fluorescence from tumour cells are reduced after setanaxib treatment (Fig. 6c).