Furthermore, based on our in silico drug sensitivity analyses and prior reports linking GNAI2 to drug resistance in other cancers [55, 66], we speculate that GNAI1 and GNAI2 overexpression could enhance chemosensitivity in COAD cells, possibly by interfering with pro-survival pathways such as PI3K/AKT [67]. This evidence concerns the gene AKT1 and colon adenocarcinoma.