TRIM6 and gastric cancer: To clarify the underlying mechanism by which TRIM6 regulates gastric tumor immunotherapy, we conducted immunoprecipitation using anti-Flag beads in MKN28 gastric cancer cell line transfected with Flag-tagged TRIM6 or a truncation mutant with deletion in the PRY-SPRY domain, which are responsible for TRIM6 substrate binding [39] (Supplementary Fig. 5a).