SDHAF2 and chronic myelogenous leukemia, BCR-ABL1 positive: We generated SDHAF2 knockouts in three different cell lines: the immortalized human embryonic kidney–derived HEK293T (human embryonic kidney–293T) cell line (17), eHAP (18), and HeLa (19) cell lines derived from chronic myelogenous leukemia and adenocarcinoma, respectively.