Here, our study reveals that KSHV and high-risk HPV31 can induce epithelial-to-mesenchymal transition by upregulation of vimentin and downregulation of E-cadherin, which is vital for KSHV-normal oral gingival keratinocyte (NOK) and HPV-NOK to acquire cancer cell characteristics such as cell survival, migration, and invasion activities. Here, VIM is linked to cancer.