Other genes were essential for multiple sarcoma cell lines, like PTK2, VPS28, and UBE2D3. We then studied the frequency of CNAs, gene fusions, and SNVs and small insertions/deletions (indels) involving each target, using publicly available DNA sequencing data (TCGA SARC [121] and MSK-IMPACT, Additional file 1:Table S13,TableS14,TableS15,TableS16) [122]. The gene discussed is UBE2D3; the disease is sarcoma.