To confirm the involvement of TRPM7 in cancer metabolism, qPCR results illustrated a down regulation of three important enzymes in KO cell line: hexokinase II (HK2), gamma enolase (ENO2) and pyruvate dehydrogenase kinase 1 (PDK1) (Fig. 2 d), which are involved in glycolysis pathway [83, 84]. This evidence concerns the gene PDK1 and cancer.