Compared with the Tg4510 tau tangle staining, the optimal excitation wavelength red-shifted from 610 nm to 660 nm, suggesting the dye L095 can in situ differentiate distinct tau conformers between Alzheimer’s disease and Tg4510 samples as we have shown for other dyes39. The gene discussed is MAPT; the disease is early-onset autosomal dominant Alzheimer disease.