We also detected cell line–specific chemotactic patterns: CD4+ T cell migration increased toward irradiated PANC-1 pancreatic cancer cells (1.39-fold, P = 0.021), whereas CD8+ T cell chemotaxis increased toward irradiated SJSA-1 osteosarcoma cells (1.26-fold, P = 0.029). Here, CD8A is linked to osteosarcoma.