To investigate whether endothelial Maf deficiency alters the composition and zonated gene expression profiles of LSEC subpopulations, we performed scRNA-seq on isolated LSECs from control and MafLSEC-KO mice followed by bioinformatic filtering to exclude contaminating ECs from large blood vessels, immune cells, stellate cells, and hepatocytes (Adgre1-, Clec4f-, Ptprc-, Cd52-, Acta1-, Arg1-, Pecam1+, Vwf-). Here, ACTA1 is linked to hyperinsulinemic hypoglycemia, familial, 4.