Second, the specific mechanism of FAM27E3 regulating p53 and its effector molecules has not been resolved; in addition, the study relies on cell line models and lacks animal in vivo lymph node metastasis experimental verification, while the immune interaction mechanism is only speculated through bioinformatics analysis and should be supplemented with tumor cell–macrophage co-culture experiments. The gene discussed is FAM27E3; the disease is neoplasm.