PTK6 and melanoma: Subsequently, CCK-8 assays (Figure 12A) and colony formation assays (Figures 12B, E) revealed that cell proliferation in the si-PTK6 group was significantly reduced in both A875 and SK-MEL-28 cell lines compared to the control (ctrl) and si-NC groups, indicating that PTK6 plays a promotive role in melanoma cell proliferation.