To target PSCA and MUC1, both TAAs known to be frequently expressed in pancreatic tumors (24, 25), we generated two human codon-optimized second generation CARs with specificity for PSCA (C.P) and MUC1 (C.M), containing CD28 and 41BB co-stimulatory endodomains, respectively, which were fused to the CD3ζ activation motif (Figure 1A) (26, 27). Here, CD28 is linked to pancreatic neoplasm.