To determine whether DAB-2-28 affects the cellular characteristics typically associated with the EMT process induced by MØ-derived factors, biochemical and biological analyses were performed to study (a) the expression of Snail1 and E-Cad, by WB; (b) cell invasion, using a Boyden chamber microinvasion assay; (c) cell motility, by a wound healing assay; (d) activation and expression of the metalloproteinase MMP9, using gelatin zymography and WB; and (e) induction of signaling pathways involved in tumor progression and EMT, by WB. This evidence concerns the gene MMP9 and neoplasm.