As expected, the results found increases in lipid distribution of the oil red O, immunofluorescence intensity of Nile red (Figure 1d,e), amount of cholesterol (Figure 2a), and even blockage of insulin signaling related glycogen storage (Figure 6c–e) were markedly elevated in the OA-stimulated hepatocytes, representing the hepatic dysmetabolism of MASLD model in vitro was successfully identified. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.