In the current study, HCT-116 had high IC50 values for PRO, which might be due to the following: it is a Duke’s stage D metastatic carcinoma [13]; it is homogenous; and it has a mut-K-RAS oncogene (G13D) [26,27], mut PIK3CA (H1047R), epigenetic alterations, high MSI, and the fastest duplication time (18 h) among all cell lines [28]. The gene discussed is PIK3CA; the disease is metastatic carcinoma.