Zebrafish oocytes of the Tg(cdh17:GFP;wt1b:GFP) line were injected with 0.15 mM nphp1-MO and 0.25 mM nphp4-MO [40], resulting in a robust phenotype characterized by glomerular cyst formation at 48 hpf (Figure 1a,b). Here, NPHP1 is linked to cyst.