This study focused on investigating the effects of a 16-week intervention with DHA-rich omega-3 PUFA supplementation and/or a progressive RT program on the circulating levels of four myokines (IL-6, METRNL, myostatin, and irisin), the cytokine TNF-α, and on certain parameters of body composition, muscle function, and serum markers of glucose and lipid metabolism in postmenopausal women with overweight or obesity. The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.