Briefly, exosomes isolated from melanoma cell lines were captured on flow cytometry beads coated with an anti-CSPG4 antibody, then surface expression of CSPG4 on these beads was bound with melanoma exosomes overnight and detected using a secondary, non-competing anti-CSPG4 antibody recognizing a distinct epitope, as per the reported technique [32]. The gene discussed is CSPG4; the disease is melanoma.