CCNE1 and colonic neoplasm: We conducted comprehensive comparisons of transformation detection capacity across various established cancer cell lines (A549 lung cancer, MDA-MB-231 triple-negative breast cancer, HCT116 colon cancer) and ovarian cancer-relevant lines (OVSAHO high-grade serous ovarian cancer, FT282-CCNE1, and TY fallopian tube epithelial cells) (Figure 3).