Strong HMGB1 immunostaining was linked to nodal metastases in high-grade serous ovarian carcinomas (p = 0.0213) and colorectal cancers (p = 0.0137), as well as to high histological grade in a combined analysis of squamous cell carcinomas originating from 11 different organs (p = 0.0010). Here, HMGB1 is linked to colorectal cancer.