The advocates of using separate cut-off values for males and females in stimulation tests had a more acceptable ratio of males to females [24,32], while in our study, the demographic data is based on only 36 cases with moderate calcitonin elevation, representing just 13.2% of all confirmed MTC cases in our clinic, with a significant dominance of female patients (86.1%), which influenced the mean values, and restricts the use of gender-specific Ct cut-offs. The gene discussed is CALCA; the disease is medullary thyroid gland carcinoma.