examined miRNAs in circulating EVs (separated by differential centrifugation and ExoQuick kit) from prediabetic patients, before following up in those that developed T2DM within 4 years, showing that miR‐10b and miR‐223‐3p, associated with insulin signaling, function as biomarkers of transition from prediabetes to T2DM [58]. This evidence concerns the gene INS and type 2 diabetes mellitus.