BRCA2 and neoplasm: For instance, for CRUK0048, a LUAD tumour with 11 clones, ALPACA inferred LOH affecting 13q12.12–13q14.3 (encompassing BRCA2, PDS5B and RB1) to occur in primary tumour clone 2, before seeding a recurrence metastasis lesion (Fig. 3a).