To examine how synthetic signalling shapes the functionality of CD8+ tumour-infiltrating lymphocytes (TILs), we performed single-cell RNA-seq (scRNA-seq) analysis of 29,008 CD8+ TILs from four conditions with potent anti-tumour efficacy: o4R, o20R, o22R and oGCSFR pmel-1 T cells, and non-transduced (NT) control T cells (Fig. 3a,b). The gene discussed is CD8A; the disease is neoplasm.