In conclusion, this two-decade retrospective analysis of genetic testing for pathogenic variants in the TTR gene highlights the significant prevalence of the V30M variant and ATTRv amyloidosis in the Balearic Islands, particularly in Mallorca and Menorca, identifying the region as potentially the third largest global focus for V30M cases after certain Swedish and Portuguese regions. This evidence concerns the gene TTR and familial amyloid neuropathy.