ERBB2 and neoplasm: Furthermore, tumor content LoD studies for MSI and TMB found reliable detection of these biomarkers at 20% cutoff (Figure 4C, 4D), which exceeded the tumor content LoD for other alterations (15% for ERBB2 CNAs, 10.5% for all SNVs and INDELs, and 12.0% for CDx variants) (Supplementary Table 7).