In vitro tests on a mouse monocyte/macrophage cell line revealed that the ozonated nanohydrogel significantly reduced the levels of vascular endothelial growth factor (VEGF, a protein highly expressed in osteoarthritis, promoting angiogenesis and cartilage degeneration), pro-inflammatory markers IL-1β, IL-6, TNF-α, and inducible nitric oxide synthase (iNOS). This evidence concerns the gene NOS2 and osteoarthritis.