Determining the expression levels of IL-1β protein in human brain tissue samples from AD 4,4 carriers for comparison to AD 3,3 and AMC 3,3, we found that the levels of IL-1β were higher in AD 4,4 brains than in either AD 3,3 or AMC brains (Figure 1A,B), as the mRNA levels of IL-1β were greater in the brains of AD 4,4 (p-0.0006) carriers compared to that in either AD 3,3 (p-0.005) or AMC carriers (Figure 1C). This evidence concerns the gene IL1B and Alzheimer disease.