Our data suggest that CX3CL1 plays a key role in regulating neuroinflammatory signaling in acquired Toxoplasmosis, highlighting its potential to prevent the neurocognitive damage observed in infected individuals.<h4>Importance</h4><i>Toxoplasma gondii</i> is a widespread parasite that forms latent cysts in neurons during chronic brain infection. Here, CX3CL1 is linked to toxoplasmosis.