To further validate the role of PVT glutamatergic neurons in anesthesia‐induced memory impairment, we injected either AAV‐EF1a‐DIO‐eYFP or AAV‐EF1a‐DIO‐NpHR‐eYFP into the PVT of Vglut2‐Cre control mice (Figure 4I,J). This evidence concerns the gene SLC17A6 and memory impairment.