showed in vitro experiments that miR-516b-5p derived from urinary exosomes of DN patients was significantly upregulated, with increased expression of IL-18 and IL-1β, as well as enhanced activity of Caspase-1 and NLRP3, the SIRT3/AMPK signaling pathway was inactivated; however, these effects were partially reversed by silencing miR-516b-5p (130). This evidence concerns the gene SIRT3 and liver dysplastic nodule.