Using ScRNA seq data (GSE146771) of CRC pre-clinical model, we found that NECTIN-2 and PVR, the two primary ligands of TIGIT, are predominantly expressed by stromal cells, especially endothelial and fibroblast populations (16, 20, 23), particularly endothelial and fibroblast cells (Figures 2A, B). Here, TIGIT is linked to colorectal carcinoma.