To confirm whether Gal3 promotes renal fibrosis mainly through the regulation of the TGFβ1 signaling pathway, we treated renal fibroblasts with Gal3 and TGFβ1, along with either the TGFBR2 inhibitor (LY2109761) or the TGFβ1 signaling pathway inhibitor (PD169316). Here, TGFB1 is linked to renal fibrosis.