Our results revealed that KLF1 silencing led to decreased A549 cell proliferation, colony formation, and invasion, whereas overexpression of KLF1 led to improved cell proliferation and invasion (p < 0.01, Figure 2C–E), suggesting that KLF1 is overexpressed in NSCLC and that it enhances NSCLC cell proliferation and invasion. Here, KLF1 is linked to non-small cell lung carcinoma.