The effects of opening and closing six-qi acupuncture (OCSQA) as an adjuvant therapy of hypnotics (i.e., Estazolam) treatment for sleep disorder of elderly were explored based on the Pittsburgh sleep quality index scores, the traditional Chinese medicine syndrome scores, the serum levels of immunoglobulins (IgG, IgA, and IgM) and the overall efficacy. This evidence concerns the gene CD40LG and sleep disorder.